PAN Olsztyn

perfusion of small intestine, used in the evaluation of bioavailability of selected components and their effect on the absorptive function of intestines,

determination of the activity of selected endogenous enzymes (saccharose, maltase, lactase, aminopeptidase) and microbial enzymes (α- and β-glucosidases, α- and β-galactosidases and β-glucuronidase) as well as products of the fermentation process (VFAs, ammonia) in intestinal digesta,

determination of biomarkers of metabolism and health of animals, including antioxidative status of the body (among others, analysis of biochemical and enzymatic blood markers),

application of pharmacological techniques in assays of functional properties of dietary constituents, using induced diabetes and colitis.

Unicam Helios α spectroscope (determination of the activity of selected endogenous and microbiological enzymes in intestinal digesta);

Asys UMV340 plate reader (immunochemical assays, possibility of measurements taken every 1 nm in a wavelength range of 340 to 800 nm);

Reflotron Plus (quantitative determination of biochemical blood markers using dry phase tests);

Odyssey – a laser system for detection and analysis of fluorescent objects in infrared (quantitative and qualitative analysis of membranes made with Western blot technique with the feasibly of results analysis using In-cell Western and On-cell Western methods);

Air-conditioned facilities of the Animal Laboratory with complete equipment for maintenance of a mother herd and for conducting studies with 120 individually-kept rats and 36 rats kept in metabolic cages.