PAN Olsztyn

Studies conducted by Embryo Biology Team concern issues related to the embryonic development in pre- and periimplantation period of oocytes quality in regard to the future embryo development. One of the studied parameters was the expression of apoptosis markers in oocytes. Moreover, antiapoptic influence of insulin-like growth factor 1 (IGF-1) was indicated in the maturation system in vitro. In addition, we investigate the influence of assisted reproductive techniques, such as different hormonal protocols of oestrus stimulation in the pig, on oocytes and embryos quality. Recently our interest has been also focused on the interactions between the embryo and uterine structures during early pregnancy. An LPA3 receptor transcript fragment sequence in pig was identified and its highest expression during periimplantation period was stated. These results confirm the contribution of lysophosphatidic acid receptors in evenly-spread embryos in pig uterus, which undoubtedly indicates its significance in the proper implantation. Moreover, the other studies focus on the influence of embryos and embryo-originated factors on the maternal recognition of pregnancy mechanism and the protection of corpus luteum against luteolysis.

Research activities of Molecular Andrology Team are concentrated on isolation, physicochemical and kinetic characterization of sperm and seminal plasma hydrolytic enzymes of fish and mammals. Investigations conducted at the Team concern genetic and environmental influence on the sperm quality parameters of teleost fish (rainbow trout, common carp, sander, Danube salmon) and sturgeons (Siberian sturgeon, starlet) as well as deer and European bison. An important direction of research is improving sperm cryopreservation methods of valuable and endangered animals. Researches in our Team lead to the improvement of effectiveness of rainbow trout and common carp sperm cryopreservation and practical application of this method in fish breeding. Cryopreservation methods of endangered animal species from the Red Book (European bison, Danube salmon) are under improvement.