PAN Olsztyn

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Achievements

  1. Elaboration of research model in vivo for investigation of interactions the embryo and uterus interactions in early stages of pregnancy and determination of hormonal profiles typical of this period

  2. Sequencing and deposition in a gene bank porcine LPA3 gene receptor transcript and determination of its expression profile during pregnancy and estrous cycle

  3. Demonstration on antiapoptic effect of IGF-1 of oocytes maturation in vitro.

  1. Isolation and characterization of serine proteinase inhibitors of fish seminal plasma. Major inhibitors of carp and rainbow trout seminal plasma have been isolated and purified. Physicochemical properties of preparations were determined, such as molecular weight, isoelectric point and glycoprotein nature of their structure. Sequence and parameters analysis allowed identifying these inhibitors as serpin of α1-antiproteinase type. The inhibitors are probably involved in the protection of spermatozoa and tissues of reproductive track against proteolytic attack, for example from microorganisms.

  2. Identification of major proteins of carp seminal plasma. Transferrin and serine proteinase inhibitors have been found as major proteins of seminal plasma for the first time for fish. An efficient method for the isolation of transferrin (Tf) has been elaborated and polyclonal antibodies for this protein have been obtained. A relationship between transferrin polymorphism and CASA parameters has been found, which indicates involvement of Tf in the mechanism of sperm motility. These proteins are part of the non-specific humoral defense mechanism. Therefore, it may be suggested that the role of seminal plasma proteins is the protection of spermatozoa against the proteolytic attack.

  3. Ovarian fluid has been found to play a significant role in motility enhancement and pH was identified as a primary determinant of this role.

  4. Characterization of proteolytic enzymes and serine proteinase inhibitor of turkey seminal plasma has been elaborated. By using electrophoretic methods the presence of two metalloproteinases, six serine proteinases and three serine proteinase inhibitors has been demonstrated in turkey seminal plasma. Metalloproteinases and the inhibitor of low electrophoretic migration rate appeared to be present both in blood and the reproductive system of birds. Serine proteinases and inhibitors with moderate and fast migration rate were present in the reproductive system only. Main inhibitors of seminal plasma belong to the Kazal family. Serine proteinase inhibitors may be involved in the inactivation of serine proteinases (including acrosin) and therefore may be capable of protecting spermatozoa and reproductive tissues against proteolytic degradation. Inhibitors of Kazal family have been isolated and characterized and cDNA sequence of acrosin has been determined.

  5. Research on the improvement of breeding and reproduction methods of black and wood grouse has been conducted to determine chances for introduction of these birds to the environment without loss of their biodiversity. Semen characteristics of black and wood grouse has been obtained for the first time.

Applied research

Most of the studies were feasible through close cooperation with fish farmers. A simple test based on analysis of water turbidity for estimation of the quality of rainbow trout eggs has been elaborated. Modification of the fertilization procedure has been proposed in order to increase the effectiveness of fertilization. Regarding experiments aimed at improving sperm quality evaluation, usefulness of the comet assay for estimation of fish sperm DNA fragmentation has been demonstrated. These results are important for the optimization of genome manipulations in fish, such as gynogenesis (production of progeny from maternal DNA).

  1. Elaborating methods of acrosin activity measurement in the sperm of boar, turkey and sturgeon without the need of extraction procedure

  2. Determination of  the influence of methylxanthines on boar spermatozoa and seminal plasma alkaline phosphatase activity and elaborating effective cryopreservation methods of sturgeon sperm.

  3. Our lab was the first which isolate and characterize arylsulphatase from Russian sturgeon and acid phosphatase from spermatozoa of rainbow trout Siberian sturgeon.

  4. As a result of achievements accomplished by the Department in fish sperm cryopreservation improvement, in 1999 the Institute has been chosen as a place of establishing the Polish Fish Sperm Bank founded by the Ministry of Agriculture and Rural Development.

Scientific achievements of the Department Staff hale been appreciated with national awards as well as Institute Director’s awards and Rector’s of the University of Warmia and Mazury in Olsztyn.

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Division of Reproductive Biology

Bydgoska 7 Str., 10-243 Olsztyn

tel. +48 89 535 74 22

fax. + 48 89 535 74 21

Department of Gamete and Embryo Biology

Head

Andrzej Ciereszko, Prof.


Embryo Biology Team

Marek Bogacki, Ph. D.

Assistant Professors

Beenu Moza Jalali, Ph.D.

Teresa Więsak, Ph.D.

Asistant

Marta Wasielak, Ph.D.

Senior Technologist

Michał Blitek, M. Sc.

Specialist

Małgorzata Karasim, M. Sc.

Ph.D. Student

Anna Kitewska, M.Sc.

 

Semen Biology Team

Assistant Professors

Mariola Dietrich, Ph. D.

Mariola Słowińska, Ph. D.

Grzegorz Janusz Dietrich, Ph. D.

Joanna Nynca, Ph.D.

Technologists

Halina Karol, Eng.

Ewa Liszewska, M. Sc.

 

Molecular Andrology Team

Professor

Jan Glogowski, Prof.

Assistant Professors

Zygmunt Giżejewski, Ph.D., D.Sc.

Beata Sarosiek, Ph.D.

Radosław Kajetan Kowalski, Ph.D.

Assistant

Beata Irena Cejko, Ph. D.

Technologists

Wiesław Jerzy Demianowicz, DVM, Ph.D.

Katarzyna Dryl, M. Sc.

Sylwia Judycka, M.Sc.

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