PAN Olsztyn

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Equipment and applied methods

  1. Fast protein liquid chromatography system (FPLC system)
    Isolation of proteins and peptides from vertebrate sperm, determination of the main physico-chemical properties of proteins.
  2. High-performance liquid chromatography system (HPLC system)
    Isolation of proteins and peptides, separation of peptides obtained after protein digestion, preparation of products for sequencing and mass spectrometry.
  3. Spectrophotometer Beckman DU 640, portable spectrophotometer Eppendorf
    Measurement of sperm concentration, an indication of the basic biochemical determinants of semen quality, measurement of antioxidant activity of the semen, kinetic analysis (determination of enzyme and inhibitor concentration, measurement of the equilibrium association constant for inhibitor - proteinase).
  4. Apparatus for measuring sperm concentration and viability (NucleoCounter SP-100)
    Measurement of sperm concentration and viability.
  5. An integrated system for the comet assay
    Assessment of sperm genome.
  6. Set vertical electrophoresis
    Visualization of seminal plasma protein profiles.
  7. Apparatus for transfer
    Transfer of proteins on the membrane, Western blot analysis.
  8. Osmometer
    Measurement of seminal plasma osmotic pressure.
  9. Additional equipment
    • Centrifuge with cooling,
    • Ultra low temperature freezer.

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Division of Reproductive Biology

Bydgoska 7 Str., 10-243 Olsztyn

tel. +48 89 535 74 22

fax. + 48 89 535 74 21

Department of Gamete and Embryo Biology

Head

Andrzej Ciereszko, Prof.


Embryo Biology Team

Marek Bogacki, Ph. D.

Assistant Professors

Beenu Moza Jalali, Ph.D.

Teresa Więsak, Ph.D.

Asistant

Marta Wasielak, Ph.D.

Senior Technologist

Michał Blitek, M. Sc.

Specialist

Małgorzata Karasim, M. Sc.

Ph.D. Student

Anna Kitewska, M.Sc.

 

Semen Biology Team

Assistant Professors

Mariola Dietrich, Ph. D.

Mariola Słowińska, Ph. D.

Grzegorz Janusz Dietrich, Ph. D.

Joanna Nynca, Ph.D.

Technologists

Halina Karol, Eng.

Ewa Liszewska, M. Sc.

 

Molecular Andrology Team

Professor

Jan Glogowski, Prof.

Assistant Professors

Zygmunt Giżejewski, Ph.D., D.Sc.

Beata Sarosiek, Ph.D.

Radosław Kajetan Kowalski, Ph.D.

Assistant

Beata Irena Cejko, Ph. D.

Technologists

Wiesław Jerzy Demianowicz, DVM, Ph.D.

Katarzyna Dryl, M. Sc.

Sylwia Judycka, M.Sc.

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