Equipment and applied methods

Preparation of the material for the studies:

The isolation of cells of corpus luteum, myometrium, endometrium and epithelium of oviduct
The cell cultures and strip incubation: an incubator (Memmert, Germany)

Analytical methods:

Viability of cells determination (cytotoxicity test): ELISA (Multiscan EX, Labsystems, Finland)
Enzyme immunoassay of steroids, protein hormones and prostaglandins (ELISA, Multiscan EX, Labsystems, Finland)
PCR and its modifications: RT-PCR, nested-PCR, touchdown PCR
RT PCR and Real-time PCR:
- RNA isolation (spectrophotometer BioPhotometr, Eppendorf, and Nonodrop)
- Reverse transcription, PCR (termocycler MJ Mini, BioRad)
- Real-time PCR (access to: ABI Prism 7300, Applied Biosystems, USA)
- Gel documentation system for electrophoresis gels (MiniBIS Pro, DNR Bio Imaging System, Israel)
5’ and 3’ RACE PCR - Rapid Amplification of cDNA Ends PCR
Western Blot analysis (Mini PROTEAN 3 System)
Intracellular Ca2+ mobilization using Fura 2 (microscope Nikon Eclipse TS 100 NIS-Elements Basic Research software)
Immunohistochemistry (access to: microscope Imager Z1-Zeiss, Axio Vision 4.8 software)
Measurement of contractility of myometrial and oviductal strips: isometric contraction transducer (access to: the HSE Type 372, Hugo Sachs Elektronik, Germany)
Determination of catecholamine concentrations by HPLC with electrochemical detection (Hewlett-Packard, Series 1050)

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Bydgoska 7, 10-243 Olsztyn

tel. +48 89 535 74 22

fax +48 89 535 74 21

Head

Jan Kotwica, Ph.D., D.Sc.

Associated Professor

Magdalena Kowalik, Ph.D.

Robert Rękawiecki, Ph.D.

Jarosław Młynarczuk, Ph.D., DVM

Michał Wróbel, Ph.D.

Senior Specialists

Beata Czarkowska, M.Sc.

Beata Ustjanowska, M.Sc.

Technicians

Beata Jarmicka

PAN Olsztyn