DEPARTMENT OF MOLECULAR ANDROLOGY

ADDRESS: Bydgoska 1/8, 10-243 Olsztyn,
tel. (+48 89) 535-74-25, fax (+48-89) 535-74-38; e-mail: glogo@pan.olsztyn.pl

  STAFF

 
Head:
  • Jan Glogowski, Prof. Ph.D.
    Prof. Assistant:
  • Zygmunt Giżejewski, D.Sc.
  • Beata Sarosiek, Ph.D.
  • Wiesław Demianowicz, Ph.D.
  • Radosław K. Kowalski, Ph.D.
    Technologist:
  • Dorota Kubiak, Ing.
    Technician:
  • Ryszard Bartecki
    Ph.D Students:
  • Grzegorz Dietrich, M.Sc.

     

    		•
     

    Head of Department, prof. Jan Glogowski.
    Field tests in hatchery often require a affected way of working.

     

      GENERAL RESEARCH PROFILE

     

        Research activity of the Department is oriented toward isolation, purification and physico-chemical and kinetic characterization of sperm hydrolytic enzymes of fish, mammals, and birds.      We also conduct studies on effects of environmental factors on the quality and biochemical parameters of turkey and boar sperm.
         In cooperation with the Department of Semen Biology practical experiments are carried out concerning improvement of sperm cryopreservation and genomic manipulation.
         As a result of this research, our Institute received a grant from the Ministry of Agriculture and Rural Development for creation and maintaince of Fish Sperm Bank.

     

     

    The staff members of the department at Fish Sperm Bank.

     

      CURRENT INVESTIGATIONS

     

     
    • Characteristics of proteolytic activity of fish seminal plasma;
    • Acrosomal sperm of sturgeon and anacrosomal sperm of rainbow trout as a model for analysis of animals acrosomal enzymes;
    • Effect of zinc level in diets on reproductive efficiency of turkey and biochemical characteristics of semen.

       
     


    PhD student Grzegorz Dietrich during work. On the picture is seen Computer assisted sperm analysis (CASA) system for measuring sperm motility (Hobson Vision - Sperm Tracker System).

     

      SELECTED PUBLICATIONS

     


    1. Glogowski J., Babiak I., Luczynski M.J., Luczynski M. (1997). Factors affecting cryopreservation efficiency and enzyme activity in northern pike, Esox lucius, sperm. J. Appl. Aquacult., 7(4): 53 - 67.
    2. Babiak I., Glogowski J., Brzuska E., Szumiec J., Adamek J. (1997). Cryopreservation of sperm of common carp, Cyprinus carpio L. Aquacult. Res., 28: 567 - 571.
    3. Glogowski J., Demianowicz W., Piros B., Ciereszko A. (1998). Determination of acrosin activity of boar spermatozoa by the clinical method: optimization of the assay and changes during short-term storage of semen. Theriogenology, 50 (6): 861 - 872.
    4. Babiak I., Glogowski J., Luczynski M.J., Luczynski M., Demianowicz W. (1999). The effect of egg yolk, low density lipoproteins, methylksanthines and fertilization diluent on cryopreservation efficiency of northern pike (Esox lucius) spermatozoa. Theriogenology, 52: 473 - 479.
    5. Tiersch T.R., Mazik P.M. (Editors). (2000). Cryopreservation in Aquatic Species. World Aquaculture Society, Baton Rouge, Louisiana. Ciereszko A., Glogowski J., Dabrowski K. Biochemical characteristics of seminal plasma and spermatozoa of freshwater fish and the relation to semen biology, quality and cryopreservation. Pp. 20 - 48.
    6. Glogowski J., Jankowski J., Faruga A., Ottobre J.S., Ciereszko A. (2001). Acrosin activity in turkey spermatozoa assay by clinical method and effect of zinc and benzamidine on the activity. Theriogenology, 56 (5): 889-901.
    7. Babiak I., Glogowski J., Goryczko K., Dobosz S., Kuzminski H. (2001). Effect of extender composition and equilibration time on fertilization ability and enzymatic activity of rainbow trout cryopreserved spermatozoa. Theriogenology, 56: 177-192.
    8. Babiak I., Glogowski J., Dobosz S., Kuzminski H., Goryczko K. (2002). Semen from rainbow trout produced using cryopreserwed spermatozoa is more suitable for cryopreservation. J. Fish Biol., 60: 561-570.
    9. Glogowski J., Kolman R., Szczepkowski M., Horvath A., Urbanyi B., Sieczyński P., Rzemieniecki A., Domagała J., Demianowicz W., Kowalski R., Ciereszko A. (2002). Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol. Aquaculture, 211: 367-373.
    10. Glogowski J., Danforth D.R., Ciereszko A. (2002). Inhibition of alkaline phosphatase activity of boar semen by pentoxifyline, caffeine, and theophylline. J. Androl., 23 (6): 783-792.
    11. Jankowski J., Glogowski J., Suszyńska D., Demianowicz W., Koncicki A., Ciereszko A. (2002). Wpływ cynku dodanego do paszy w zróżnicowanych dawkach i postaci na jakość nasienia indorów. Medycyna Wet., 58 (11): 895-898.
    12. Piros B., Glogowski J., Kolman R., Rzemieniecki A., Domagala J., Horvath A., Urbanyi B., Ciereszko A. (2002). Biochemical characterization of Siberian sturgeon Acipenser baeri and sterlet Acipenser ruthenus milt plasma and spermatozoa. Fish Physiol., Biochem., 26 (3): 289-295.
    13. Kowalski R., Glogowski J., Kucharczyk D., Goryczko K., Dobosz S., Ciereszko A. (2003). Proteolytic activity and electrophoretic profiles of proteases from seminal plasma of teleost. J. Fish Biol., 62: 1008-1019.
    14. Kowalski R., Wojtczak M., Glogowski J., Ciereszko A. (2003). Gelatinolytic and anti-trypsin activities in seminal plasma of common carp: relationship to blood, skin mucus and spermatozoa. Aquat. Living Res., 16: 438-444.
    15. Rzemieniecki A., Domagała J., Glogowski J., Ciereszko A., Trzebiatowski R., Kouril J., Hamackova J., Babiak I. (2004). Induced spermiation in 3-year-old sterlet, Acipoenser ruthenus L. Aquacult. Res., 35: 144-151.
    16. Kowalski R., Glogowski J., Kucharczyk D., Mak M., Dobosz S., Zakes Z. and Ciereszko A. (2004). Characterization of gelatinolytic activity in seminal plasma of some teleost fish. Aquaculture International, in press.
    17. Sarosiek B., Ciereszko A., Rzemieniecki A., Domagała J., Glogowski J. (2004). The influence of semen cryopreservation on release of some enzymes from Siberian sturgeon (Acipenser baeri) and sterlet (Acipenser ruthenus) spermatozoa. Archs. Polish Fish., in press.

     

     

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